The neuropilin-1 receptor mediates enhanced tumor delivery of H2K polyplexes.

ABSTRACT:

Background

Promising plasmid-based treatments have limited value without an effective delivery system. Recently, the linear H2K with a repeating -KHHK- pattern was determined to be an effective plasmid carrier to tumor xenografts in vivo. Although unpacking of the H2K polyplex within the tumor may have a role, the mechanism for the enhanced efficacy remains unclear.

Methods

After solid-phase synthesis of linear and branched histidine-lysine (HK) peptide carriers of plasmids, the peptides were compared for their ability to lyse endosomes with a red blood cell model and to transfect MDA-MB-435 xenografts in the presence or absence of neuropilin-1 receptor (NRP-1) antibodies. To examine stability, polyplexes were incubated with trypsin or NaCl and then analyzed by electrophores

SUBMITTER: Leng Q 

PROVIDER: S-EPMC4948292 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature