Regulation of matrix metalloproteinase expression by dynamic tensile strain in rat fibrochondrocytes.

ABSTRACT:

Objective

We sought to determine the molecular basis for the anticatabolic effects of mechanical signals on fibrocartilage cells by studying the expression of a variety of matrix metalloproteinases (MMPs). Furthermore, we examined whether the effects of biomechanical strain on MMP gene expression are sustained.

Methods

Fibrochondrocytes from temporomandibular joint (TMJ) discs were exposed to dynamic tensile strain for various time intervals in the presence of interleukin (IL)-1beta. The regulation of the messenger RNA (mRNA) expression and synthesis of MMPs and tissue inhibitors of MMPs (TIMPs) were examined by end-point and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) as well as Western blot analysis.

Results

Fibrochondrocytes expressed mRNA fo

SUBMITTER: Deschner J 

PROVIDER: S-EPMC4948984 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature