Reference genes for accurate gene expression analyses across different tissues, developmental stages and genotypes in rice for drought tolerance.

ABSTRACT:

Background

Quantitative reverse transcription PCR (qRT-PCR) has been routinely used to quantify gene expression level. This technique determines the expression of a target gene by comparison to an internal control gene uniformly expressed among the samples analyzed. The reproducibility and reliability of the results depend heavily on the reference genes used. To achieve successful gene expression analyses for drought tolerance studies in rice, reference gene selection should be based on consistency in expression across variables. We aimed to provide reference genes that would be consistent across different tissues, developmental stages and genotypes of rice and hence improve the quality of data in qRT-PCR analysis.

Findings

Ten candidate reference genes were screened from fo

SUBMITTER: Pabuayon IM 

PROVIDER: S-EPMC4949181 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature