A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization.

ABSTRACT: The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. In addition, their cell size, morphology and division rate, and low expression of native channels are traits that are particularly attractive for current-voltage measurements. Nevertheless, the heterologous expression of complex membrane proteins such as receptors and ion channels for biological characterization and in particular for single-cell applications such as electrophysiology remains a challenge. Expression of functional proteins depends largely on careful step-by-step optimization that includes the design of expression vectors with suitable identificati

SUBMITTER: Ooi A 

PROVIDER: S-EPMC4949579 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature