Molecular Buffers Permit Sensitivity Tuning and Inversion of Riboswitch Signals.

ABSTRACT: Predictable integration of foreign biological signals and parts remains a key challenge in the systematic engineering of synthetic cellular actuations, and general methods to improve signal transduction and sensitivity are needed. To address this problem we modeled and built a molecular signal buffer network in Saccharomyces cerevisiae inspired by chemical pH buffer systems. The molecular buffer system context-insulates a riboswitch enabling synthetic control of colony formation and modular signal manipulations. The riboswitch signal is relayed to a transcriptional activation domain of a split transcription factor, while interacting DNA-binding domains mediate the transduction of signal and form an interacting molecular buffer. The molecular buffer system enables modular signal inversion t

SUBMITTER: Rugbjerg P 

PROVIDER: S-EPMC4949582 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature