Highly Multiplexed RNA Aptamer Selection using a Microplate-based Microcolumn Device.

ABSTRACT: We describe a multiplexed RNA aptamer selection to 19 different targets simultaneously using a microcolumn-based device, MEDUSA (Microplate-based Enrichment Device Used for the Selection of Aptamers), as well as a modified selection process, that significantly reduce the time and reagents needed for selections. We exploited MEDUSA's reconfigurable design between parallel and serially-connected microcolumns to enable the use of just 2 aliquots of starting library, and its 96-well microplate compatibility to enable the continued use of high-throughput techniques in downstream processes. Our modified selection protocol allowed us to perform the equivalent of a 10-cycle selection in the time it takes for 4 traditional selection cycles. Several aptamers were discovered with nanomolar dissociati

SUBMITTER: Reinholt SJ 

PROVIDER: S-EPMC4949599 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature