Complex polar machinery required for proper chromosome segregation in vegetative and sporulating cells of Bacillus subtilis.

ABSTRACT: Chromosome segregation is an essential process of cell multiplication. In prokaryotes, segregation starts with the newly replicated sister origins of replication, oriCs, which move apart to defined positions in the cell. We have developed a genetic screen to identify mutants defective in placement of oriC during spore development in the Gram-positive bacterium Bacillus subtilis. In addition to the previously identified proteins Soj and DivIVA, our screen identified several new factors involved in polar recruitment of oriC: a reported regulator of competence ComN, and the regulators of division site selection MinD and MinJ. Previous work implicated Soj as an important regulator of oriC positioning in the cell. Our results suggest a model in which the DivIVA-interacting proteins ComN and Min

SUBMITTER: Kloosterman TG 

PROVIDER: S-EPMC4949633 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature