Increased expression of HIF2α during iron deficiency-associated megakaryocytic differentiation.

ABSTRACT:

Background

Iron deficiency is associated with reactive thrombocytosis; however, the mechanisms driving this phenomenon remain unclear. We previously demonstrated that this occurs alongside enhanced megakaryopoiesis in iron-deficient rats, without alterations in the megakaryopoietic growth factors thrombopoietin, interleukin-6, or interleukin-11.

Objectives

The aim of this study was to evaluate megakaryocyte differentiation under iron deficiency in an in vitro model and to investigate potential genes involved in this process.

Methods

Human erythroleukemia and megakaryoblastic leukemia cell lines, as well as cord-blood derived hematopoietic stem cells were cultured under iron deficiency. Cell morphology, ploidy, expression of CD41, CD61, and CD42b, and proplatelet forma

SUBMITTER: Jimenez K 

PROVIDER: S-EPMC4949661 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature