Protein adsorption onto nanoparticles induces conformational changes: Particle size dependency, kinetics, and mechanisms.

ABSTRACT: The use of nanomaterials in bioapplications demands a detailed understanding of protein-nanoparticle interactions. Proteins can undergo conformational changes while adsorbing onto nanoparticles, but studies on the impact of particle size on conformational changes are scarce. We have shown that conformational changes happening upon adsorption of myoglobin and BSA are dependent on the size of the nanoparticle they are adsorbing to. Out of eight initially investigated model proteins, two (BSA and myoglobin) showed conformational changes, and in both cases this conformational change was dependent on the size of the nanoparticle. Nanoparticle sizes ranged from 30 to 1000 nm and, in contrast to previous studies, we attempted to use a continuous progression of sizes in the range found in live vir

SUBMITTER: Satzer P 

PROVIDER: S-EPMC4949706 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature