Quantitative analysis of N-acylphosphatidylethanolamine molecular species in rat brain using solid-phase extraction combined with reversed-phase chromatography and tandem mass spectrometry.

ABSTRACT: A novel method for the sensitive and selective identification and quantification of N-acylphosphatidylethanolamine molecular species was developed. Samples were prepared using a combination of liquid-liquid and solid-phase extraction, and intact N-acylphosphatidylethanolamine species were determined by reversed-phase high-performance liquid chromatography coupled to positive electrospray tandem mass spectrometry. As a result of their biological functions as precursors for N-acylethanolamines and as signaling molecules, tissue concentrations of N-acylphosphatidylethanolamines are very low, and their analysis is additionally hindered by the vast excess of other sample components. Our sample preparation methods are able to selectively separate the analytes of interest from any expected biolog

SUBMITTER: Triebl A 

PROVIDER: S-EPMC4949747 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature