Quantification of the oxygen uptake rate in a dissolved oxygen controlled oscillating jet-driven microbioreactor.

ABSTRACT:

Background

Microbioreactors have emerged as a new tool for early bioprocess development. The technology has advanced rapidly in the last decade and obtaining real-time quantitative data of process variables is nowadays state of the art. In addition, control over process variables has also been achieved. The aim of this study was to build a microbioreactor capable of controlling dissolved oxygen (DO) concentrations and to determine oxygen uptake rate in real time.

Results

An oscillating jet driven, membrane-aerated microbioreactor was developed without comprising any moving parts. Mixing times of ∼7 s, and k_La values of ∼170 h^-1 were achieved. DO control was achieved by varying the duty cycle of a solenoid microvalve, which changed the gas mixture in the

SUBMITTER: Kirk TV 

PROVIDER: S-EPMC4950047 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature