Relationship between cytokine expression patterns and clinical outcomes: two population-based birth cohorts.

ABSTRACT:

Background

Models that incorporate patterns of multiple cytokine responses to allergens, rather than individual cytokine production, may better predict sensitization and asthma.

Objective

To characterize the patterns of peripheral blood mononuclear cells' (PBMCs) cytokine responses to house dust mite (HDM) allergens among children from two population-based birth cohorts using machine learning techniques.

Methods

PBMCs collected at 8 years of age from the UK Manchester Asthma and Allergy Study (n = 268) and at 14 years of age from the Australian Raine Study (n = 1374) were cultured with HDM extract (10 μg/ml). Cytokine expression (IL-13, IL-5, IFN-γ, and IL10) was measured in the supernatant. Cytokine patterns were identified using a Gaussian mixture model clustering,

SUBMITTER: Wu J 

PROVIDER: S-EPMC4950290 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature