Nanoallergens: A multivalent platform for studying and evaluating potency of allergen epitopes in cellular degranulation.

ABSTRACT: Degranulation caused by type I hypersensitivity (allergies) is a complex biophysical process, and available experimental models for studying relevant immunoglobulin E binding epitopes on allergen proteins lack the ability to adequately evaluate, rank, and associate these epitopes individually and with each other. In this study, we propose a new allergy model system for studying potential allergen epitopes using nanoallergens, liposomes modified to effectively display IgE binding epitopes/haptens. By utilizing the covalently conjugated lipid tails on two hapten molecules (dinitrophenol and dansyl), hapten molecules were successfully incorporated into liposomes with high precision to form nanoallergens. Nanoallergens, with precisely controlled high-particle valency, can trigger degranulation

SUBMITTER: Deak PE 

PROVIDER: S-EPMC4950352 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature