DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods.

ABSTRACT:

Background

DNA isolation and PCR amplification from molluscan taxa is considered as problematic because polysaccharides in tissue and mucus presumably co-precipitate with the DNA and inhibit the activity of DNA polymerase. In the present study we tested two common extraction methods on specimens from the mollusc collection of the Natural History Museum Vienna (NHMW). We analysed a broad variety of taxa covering a large temporal span (acquisition years 1877 to 1999), which distinguishes our study from previous ones where mostly fresh material was used. We also took other factors into account: effects of sample age, effects of formaldehyde treatment and taxon-specific problems. We used several primer combinations to amplify amplicons of different lengths of two mitochondrial genes: c

SUBMITTER: Jaksch K 

PROVIDER: S-EPMC4950716 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature