Population Genomics of Reduced Vancomycin Susceptibility in Staphylococcus aureus.

ABSTRACT: The increased prevalence of vancomycin-intermediate Staphylococcus aureus (VISA) is an emerging health care threat. Genome-based comparative methods hold great promise to uncover the genetic basis of the VISA phenotype, which remains obscure. S. aureus isolates were collected from a single individual that presented with recurrent staphylococcal bacteremia at three time points, and the isolates showed successively reduced levels of vancomycin susceptibility. A population genomic approach was taken to compare patient S. aureus isolates with decreasing vancomycin susceptibility across the three time points. To do this, patient isolates were sequenced to high coverage (~500×), and sequence reads were used to model site-specific allelic variation within and between isolate populations. Populati

SUBMITTER: Rishishwar L 

PROVIDER: S-EPMC4954867 | biostudies-literature | 2016 Jul-Aug

REPOSITORIES: biostudies-literature