Dataset Information
Inducible colistin resistance via a disrupted plasmid-borne mcr-1 gene in a 2008 Vietnamese Shigella sonnei isolate.
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ABSTRACT: Objectives
The objective of this study was to assess the presence of mcr-1 in Shigella sonnei isolated in Vietnam.Methods
WGS data were analysed for the presence of the mcr-1 gene sequence. The association of mcr-1 with a plasmid was assessed by PCR and by conjugation.Results
Through genome sequencing we identified a plasmid-associated inactive form of mcr-1 in a 2008 Vietnamese isolate of Shigella sonnei. The plasmid was conjugated into Escherichia coli and mcr-1 was activated upon exposure to colistin, resulting in highly colistin-resistant transconjugants.Conclusions
This is the first description of the mcr-1 gene in Shigella, which is atypical given that colistin is not ordinarily used to treat diarrhoea. Our data suggest the mcr-1 gene has been circulating in human-restricted pathogens for some time but likely carries a selective fitness cost.
SUBMITTER: Pham Thanh D
PROVIDER: S-EPMC4954929 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Publications
Inducible colistin resistance via a disrupted plasmid-borne mcr-1 gene in a 2008 Vietnamese Shigella sonnei isolate.
The Journal of antimicrobial chemotherapy 20160530 8
<h4>Objectives</h4>The objective of this study was to assess the presence of mcr-1 in Shigella sonnei isolated in Vietnam.<h4>Methods</h4>WGS data were analysed for the presence of the mcr-1 gene sequence. The association of mcr-1 with a plasmid was assessed by PCR and by conjugation.<h4>Results</h4>Through genome sequencing we identified a plasmid-associated inactive form of mcr-1 in a 2008 Vietnamese isolate of Shigella sonnei. The plasmid was conjugated into Escherichia coli and mcr-1 was act ...[more]