A clickable glutathione approach for identification of protein glutathionylation in response to glucose metabolism.

ABSTRACT: Glucose metabolism and mitochondrial function are closely interconnected with cellular redox-homeostasis. Although glucose starvation, which mimics ischemic conditions or insufficient vascularization, is known to perturb redox-homeostasis, global and individual protein glutathionylation in response to glucose metabolism or mitochondrial activity remains largely unknown. In this report, we use our clickable glutathione approach, which forms clickable glutathione (azido-glutathione) by using a mutant of glutathione synthetase (GS M4), for detection and identification of protein glutathionylation in response to glucose starvation. We found that protein glutathionylation is readily induced in HEK293 cells in response to low glucose concentrations when mitochondrial reactive oxygen species (ROS

SUBMITTER: Samarasinghe KT 

PROVIDER: S-EPMC4955733 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature