Removal of acetaminophen protein adducts by autophagy protects against acetaminophen-induced liver injury in mice.

ABSTRACT:

Background & aims

Acetaminophen (APAP)-induced liver injury is the most frequent cause of acute liver failure in the US and many other countries. Metabolism of APAP results in formation of APAP protein adducts (APAP-AD) in hepatocytes and triggers mitochondrial dysfunction and necrosis. However, the mechanisms for how APAP-AD are removed from hepatocytes remain unknown.

Methods

Mice or primary hepatocytes were treated with APAP. APAP-AD were determined by immunoblot, immunostaining and high pressure liquid chomatography with electrochemical detection analysis.

Results

We found that APAP-AD were detected at 1h, peaked at approximately 2h, declined at 6h and almost full removed at 24h post treatment with APAP in mouse livers and in primary mouse hepatocytes. APAP-AD dis

SUBMITTER: Ni HM 

PROVIDER: S-EPMC4955750 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature