Early changes in gene expression induced by blue light irradiation of A2E-laden retinal pigment epithelial cells.

ABSTRACT:

Purpose

Accumulation of bisretinoids as lipofuscin in retinal pigment epithelial (RPE) cells is implicated in the pathogenesis of some blinding diseases including age-related macular degeneration (AMD). To identify genes whose expression may change under conditions of bisretinoid accumulation, we investigated the differential gene expression in RPE cells that had accumulated the lipofuscin fluorophore A2E and were exposed to blue light (430 nm).

Methods

A2E-laden RPE cells were exposed to blue light (A2E/430 nm) at various time intervals. Cell death was quantified using Dead Red staining, and RNA levels for the entire genome was determined using DNA microarrays (Affymetrix GeneChip Human Genome 2.0 Plus). Array results for selected genes were confirmed by real-time reverse-t

SUBMITTER: van der Burght BW 

PROVIDER: S-EPMC4955808 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature