Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis.

ABSTRACT: Simple and rapid methods for analysis of monoclonal antibody structure and post-translational modifications are increasingly needed due to the explosion of therapeutic monoclonal antibodies and monoclonal antibody applications. Mass spectral analysis is a powerful method for characterizing monoclonal antibodies. Recent discovery and commercialization of the Immunoglobulin G-degrading enzyme of Streptococcus pyogene (IdeS protease) has facilitated and improved the generation of antibody fragments of suitable size to allow characterization of the structure of the entire antibody molecule via analysis of just a few fragments. In this study, we coupled IdeS fragmentation and simultaneous reduction and alkylation of the resultant fragments using tributylphosphine and iodoacetamide to prepare sa

SUBMITTER: Kirley TL 

PROVIDER: S-EPMC4955844 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature