A Myocardial Slice Culture Model Reveals Alpha-1A-Adrenergic Receptor Signaling in the Human Heart.

ABSTRACT:

Background

Translation of preclinical findings could benefit from a simple, reproducible, high throughput human model to study myocardial signaling. Alpha-1A-adrenergic receptors (ARs) are expressed at very low levels in the human heart, and it is unknown if they function.

Objectives

To develop a high throughput human myocardial slice culture model, and to test the hypothesis that alpha-1A- ARs are functional in the human heart.

Methods

Cores of LV free wall 8 mm diameter were taken from 52 hearts (18 failing and 34 nonfailing). Slices 250 μm thick were cut with a Krumdieck apparatus and cultured using a rotating incubation unit.

Results

About 60 slices were cut from each LV core, and a typical study could use 96 slices. Myocyte morphology was maintained, and d

SUBMITTER: Thomas RC 

PROVIDER: S-EPMC4955869 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature