Feasibility of Unbiased RNA Profiling of Colorectal Tumors: A Proof of Principle.

ABSTRACT: Despite recent advances in molecular profiling of colorectal cancer (CRC), as of yet this has not translated into an unbiased molecular liquid biopsy profile which can accurately screen for early CRC. In this study we depict the profile of early stage CRC as well as for advanced adenomas (AA) by combination of current molecular knowledge with microarray technology, using efficient circulating free plasma RNA purification from blood and RNA amplification technologies. We joined literature search with Affymetrix gene chip experimental procedure to draw the circulating free plasma RNA profile of colorectal cancer disease reflected in blood. The RNA panel was tested by two datasets comparing patients with CRC with healthy subjects and patients with AA to healthy subjects. For the CRC patient c

SUBMITTER: Moshayoff V 

PROVIDER: S-EPMC4956030 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature