Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment.

ABSTRACT: The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom microfabricated tapered microwells were integrated with microfluidics to allow robust formation of CTC clusters without pre-enrichment and subsequent drug screening in situ. Rapid feedback after 2 weeks promotes immediate intervention upon detection of drug resistance or tolerance. The procedure was clinically validated with blood samples (n = 73) from 55 patients with early-stage, newly diagnosed, locally advanced, or refractory metastatic breast cancer. Twenty-four of these samples were used for d

SUBMITTER: Khoo BL 

PROVIDER: S-EPMC4956185 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature