Heterogeneity of cellular circadian clocks in intact plants and its correction under light-dark cycles.

ABSTRACT: Recent advances in single-cell analysis have revealed the stochasticity and nongenetic heterogeneity inherent to cellular processes. However, our knowledge of the actual cellular behaviors in a living multicellular organism is still limited. By using a single-cell bioluminescence imaging technique on duckweed, Lemna gibba, we demonstrate that, under constant conditions, cells in the intact plant work as individual circadian clocks that oscillate with their own frequencies and respond independently to external stimuli. Quantitative analysis uncovered the heterogeneity and instability of cellular clocks and partial synchronization between neighboring cells. Furthermore, we found that cellular clocks in the plant body under light-dark cycles showed a centrifugal phase pattern in which the eff

SUBMITTER: Muranaka T 

PROVIDER: S-EPMC4956400 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature