Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering.

ABSTRACT: Precise genome engineering is instrumental for biomedical research and holds great promise for future therapeutic applications. Site-specific recombinases (SSRs) are valuable tools for genome engineering due to their exceptional ability to mediate precise excision, integration and inversion of genomic DNA in living systems. The ever-increasing complexity of genome manipulations and the desire to understand the DNA-binding specificity of these enzymes are driving efforts to identify novel SSR systems with unique properties. Here, we describe two novel tyrosine site-specific recombination systems designated Nigri/nox and Panto/pox. Nigri originates from Vibrio nigripulchritudo (plasmid VIBNI_pA) and recombines its target site nox with high efficiency and high target-site selectivity, without

SUBMITTER: Karimova M 

PROVIDER: S-EPMC4957104 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature