A Bayesian algorithm for detecting differentially expressed proteins and its application in breast cancer research.

ABSTRACT: Presence of considerable noise and missing data points make analysis of mass-spectrometry (MS) based proteomic data a challenging task. The missing values in MS data are caused by the inability of MS machines to reliably detect proteins whose abundances fall below the detection limit. We developed a Bayesian algorithm that exploits this knowledge and uses missing data points as a complementary source of information to the observed protein intensities in order to find differentially expressed proteins by analysing MS based proteomic data. We compared its accuracy with many other methods using several simulated datasets. It consistently outperformed other methods. We then used it to analyse proteomic screens of a breast cancer (BC) patient cohort. It revealed large differences between the pr

SUBMITTER: Santra T 

PROVIDER: S-EPMC4957118 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature