M1 Macrophages Activate Notch Signalling in Epithelial Cells: Relevance in Crohn's Disease.

ABSTRACT:

Background

The Notch signalling pathway plays an essential role in mucosal regeneration, which constitutes a key goal of Crohn's disease (CD) treatment. Macrophages coordinate tissue repair and several phenotypes have been reported which differ in the expression of surface proteins, cytokines and hypoxia-inducible factors (HIFs). We analysed the role of HIFs in the expression of Notch ligands in macrophages and the relevance of this pathway in mucosal regeneration.

Methods

Human monocytes and U937-derived macrophages were polarized towards the M1 and M2 phenotypes and the expression levels of HIF-1α, HIF-2α, Jagged 1 (Jag1) and delta-like 4 (Dll4) were evaluated. The effects of macrophages on the expression of hairy and enhancer of split-1 (HES1, the main target of Notch sig

SUBMITTER: Ortiz-Masia D 

PROVIDER: S-EPMC4957456 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature