More Easily Cultivated Than Identified: Classical Isolation With Molecular Identification of Vaginal Bacteria.

ABSTRACT:

Background

Women with bacterial vaginosis (BV) have complex communities of anaerobic bacteria. There are no cultivated isolates of several bacteria identified using molecular methods and associated with BV. It is unclear whether this is due to the inability to adequately propagate these bacteria or to correctly identify them in culture.

Methods

Vaginal fluid from 15 women was plated on 6 different media using classical cultivation approaches. Individual isolates were identified by 16S ribosomal RNA (rRNA) gene sequencing and compared with validly described species. Bacterial community profiles in vaginal samples were determined using broad-range 16S rRNA gene polymerase chain reaction and pyrosequencing.

Results

We isolated and identified 101 distinct bacterial strain

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC4957512 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature