External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015.

ABSTRACT:

Objective

To conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013.

Methods

Twenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. Module B contained human serum samples for the detection of anti-DENV antibodies.

Results

Among 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymera

SUBMITTER: Soh LT 

PROVIDER: S-EPMC4957610 | biostudies-literature | 2016 Apr-Jun

REPOSITORIES: biostudies-literature