Quantitative proteomic analysis of cell envelope preparations under iron starvation stress in Aeromonas hydrophila.

ABSTRACT:

Background

Iron homeostasis is an essential process over the entire lives of both hosts and bacterial pathogens, and also plays roles in many other metabolic functions. Currently, knowledge is limited on the iron scavenging mechanism of the cell envelope in the aquatic pathogen, Aeromonas hydrophila. To understand the iron homeostasis mechanism in A. hydrophila, a dimethyl labelling based quantitative proteomics method was used to compare the differential expression of cell envelope proteins under iron starvation.

Results

A total of 542 cell envelope proteins were identified by LC-MS/MS, with 66 down-regulated and 104 up-regulated proteins. Bioinformatics analysis showed that outer membrane siderophores, heme and iron receptors, periplasmic iron binding proteins, inner membr

SUBMITTER: Yao Z 

PROVIDER: S-EPMC4957856 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature