Biosynthesis of glycerol phosphate is associated with long-term potentiation in hippocampal neurons.

ABSTRACT:

Introduction

Neurons have a very high energy requirement, and their metabolism is tightly regulated to ensure delivery of adequate substrate to sustain neuronal activity and neuroplastic changes. The mechanisms underlying the regulation of neuronal metabolism, however, are not completely clear.

Objective

The objective of this study was to investigate the central carbon metabolism in neurons, in order to identify the regulatory pathways governing neuronal anabolism and catabolism.

Methods

Here we first have applied MS-based endometabolomics to elucidate the metabolic dynamics in cultured hippocampal primary neurons. Using nanoLC-ESI-LTQ Orbitrap MS approach followed by statistical analysis, we measure the dynamics of uniformly labeled ¹³C-glucose entering ne

SUBMITTER: Martano G 

PROVIDER: S-EPMC4958395 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature