Characterisation of the protein corona using tunable resistive pulse sensing: determining the change and distribution of a particle's surface charge.

ABSTRACT: The zeta potential of the protein corona around carboxyl particles has been measured using tunable resistive pulse sensing (TRPS). A simple and rapid assay for characterising zeta potentials within buffer, serum and plasma is presented monitoring the change, magnitude and distribution of proteins on the particle surface. First, we measure the change in zeta potential of carboxyl-functionalised nanoparticles in solutions that contain biologically relevant concentrations of individual proteins, typically constituted in plasma and serum, and observe a significant difference in distributions and zeta values between room temperature and 37 °C assays. The effect is protein dependent, and the largest difference between the two temperatures is recorded for the γ-globulin protein where the mean zet

SUBMITTER: Blundell ELCJ 

PROVIDER: S-EPMC4958399 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature