Quantitative Magnetic Separation of Particles and Cells Using Gradient Magnetic Ratcheting.

ABSTRACT: Extraction of rare target cells from biosamples is enabling for life science research. Traditional rare cell separation techniques, such as magnetic activated cell sorting, are robust but perform coarse, qualitative separations based on surface antigen expression. A quantitative magnetic separation technology is reported using high-force magnetic ratcheting over arrays of magnetically soft micropillars with gradient spacing, and the system is used to separate and concentrate magnetic beads based on iron oxide content (IOC) and cells based on surface expression. The system consists of a microchip of permalloy micropillar arrays with increasing lateral pitch and a mechatronic device to generate a cycling magnetic field. Particles with higher IOC separate and equilibrate along the miropillar

SUBMITTER: Murray C 

PROVIDER: S-EPMC4958462 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature