Mitochondrial oxidative stress during cardiac lipid overload causes intracellular calcium leak and arrhythmia.

ABSTRACT:

Background

Diabetes and obesity are associated with an increased risk of arrhythmia and sudden cardiac death. Abnormal lipid accumulation is observed in cardiomyocytes of obese and diabetic patients, which may contribute to arrhythmia, but the mechanisms are poorly understood. A transgenic mouse model of cardiac lipid overload, the peroxisome proliferator-activated receptor-γ (PPARg) cardiac overexpression mouse, has long QT and increased ventricular ectopy.

Objective

The purpose of this study was to evaluate the hypothesis that the increase in ventricular ectopy during cardiac lipid overload is caused by abnormalities in calcium handling due to increased mitochondrial oxidative stress.

Methods

Ventricular myocytes were isolated from adult mouse hearts to record spark

SUBMITTER: Joseph LC 

PROVIDER: S-EPMC4958577 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature