A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement.

ABSTRACT: Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has become an important tool for measuring distances in proteins on the order of a few nm. For this purpose pairs of spin labels, most commonly nitroxides, are site-selectively introduced into the protein. Recent efforts to develop new spin labels are focused on tailoring the intrinsic properties of the label to either extend the upper limit of measurable distances at physiological temperature, or to provide a unique spectral lineshape so that selective pairwise distances can be measured in a protein or complex containing multiple spin label species. Triarylmethyl (TAM) radicals are the foundation for a new class of spin labels that promise to provide both capabilities. Here we report

SUBMITTER: Yang Z 

PROVIDER: S-EPMC4958593 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature