Human Trefoil Factor 3 induces the transcription of its own promoter through STAT3.

ABSTRACT: Human trefoil factor 3 (hTFF3) is a small peptide of potential therapeutic value. The mechanisms underlying the transcriptional regulation of hTFF3 remain unclear. The purpose of this study was to identify the core functional elements for the self-induction action of hTFF3 and transcription factors. First, truncated promoters were constructed to identify the functional regions of the hTFF3 promoter. Next, point mutation, chromatin immunoprecipitation, RNA interference, and gene overexpression experiments were performed to analyze the transcriptional binding sites responsible for the self-induced transcription of hTFF3. Our results revealed the -1450 bp to -1400 bp fragment of the hTFF3 promoter was the functional region for the self-induction action of hTFF3. Bioinformatics analysis confir

SUBMITTER: Sun Y 

PROVIDER: S-EPMC4958921 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature