Mechanism of Attenuation of Uranyl Toxicity by Glutathione in Lactococcus lactis.

ABSTRACT:

Unlabelled

Both prokaryotic and eukaryotic organisms possess mechanisms for the detoxification of heavy metals, and these mechanisms are found among distantly related species. We investigated the role of intracellular glutathione (GSH), which, in a large number of taxa, plays a role in protection against the toxicity of common heavy metals. Anaerobically grown Lactococcus lactis containing an inducible GSH synthesis pathway was used as a model organism. Its physiological condition allowed study of putative GSH-dependent uranyl detoxification mechanisms without interference from additional reactive oxygen species. By microcalorimetric measurements of metabolic heat during cultivation, it was shown that intracellular GSH attenuates the toxicity of uranium at a concentration in the ra

SUBMITTER: Obeid MH 

PROVIDER: S-EPMC4959180 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature