Modification of a High-Throughput Automatic Microbial Cell Enumeration System for Shipboard Analyses.

ABSTRACT: In the age of ever-increasing "-omics" studies, the accurate and statistically robust determination of microbial cell numbers within often-complex samples remains a key task in microbial ecology. Microscopic quantification is still the only method to enumerate specific subgroups of microbial clades within complex communities by, for example, fluorescence in situ hybridization (FISH). In this study, we improved an existing automatic image acquisition and cell enumeration system and adapted it for usage at high seas on board an oceanographic research ship. The system was evaluated by testing settings such as minimal pixel area and image exposure times ashore under stable laboratory conditions before being brought on board and tested under various wind and wave conditions. The system was robu

SUBMITTER: Bennke CM 

PROVIDER: S-EPMC4959242 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature