Lack of KIR4.1 autoantibodies in Japanese patients with MS and NMO.

ABSTRACT:

Objectives

To examine anti-KIR4.1 antibodies by 2 different assays in Japanese patients with multiple sclerosis (MS) or neuromyelitis optica (NMO).

Methods

One hundred sixty serum samples from 57 patients with MS, 40 patients with NMO/NMO spectrum disorder (NMOSD), and 50 healthy controls (all were Japanese) were tested with ELISA using a synthetic peptide of the first extracellular portion of human KIR4.1. In addition, we attempted to detect anti-KIR4.1 immunoglobulin G in the serum by the luciferase immunoprecipitation systems (LIPS) with the full length of human KIR4.1 produced in a human cell line, which is highly sensitive to single or multiple epitopes.

Results

We failed to detect antibodies to the peptide fragment KIR4.1(83-120) in any case of MS and NMO/NMOSD

SUBMITTER: Higuchi O 

PROVIDER: S-EPMC4959509 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature