Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature.

ABSTRACT:

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Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins. This scaffoldin represented a technical limit of sorts, since adding another cohesin proved problematic, owing to resultant low expression levels, instability (cleavage) of the scaffoldin polypeptide, and limited numbers of available cohesin-dockerin specificities-the hallmark of designer cellulosomes. Nevertheless, increasing the number of enzymes integrated into designer cellulosomes is critical, in order to further enhance degradation of plant cell wall material. Adaptor scaffoldins comprise an intermediate type of scaffoldi

SUBMITTER: Stern J 

PROVIDER: S-EPMC4959524 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature