Establishment and Characterization of a Tumor Stem Cell-Based Glioblastoma Invasion Model.

ABSTRACT:

Aims

Glioblastoma is the most frequent and malignant brain tumor. Recurrence is inevitable and most likely connected to tumor invasion and presence of therapy resistant stem-like tumor cells. The aim was therefore to establish and characterize a three-dimensional in vivo-like in vitro model taking invasion and tumor stemness into account.

Methods

Glioblastoma stem cell-like containing spheroid (GSS) cultures derived from three different patients were established and characterized. The spheroids were implanted in vitro into rat brain slice cultures grown in stem cell medium and in vivo into brains of immuno-compromised mice. Invasion was followed in the slice cultures by confocal time-lapse microscopy. Using immunohistochemistry, we compared tumor cell invasion as well as exp

SUBMITTER: Jensen SS 

PROVIDER: S-EPMC4959755 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature