The Photosensitivity of Rhodopsin Bleaching and Light-Induced Increases of Fundus Reflectance in Mice Measured In Vivo With Scanning Laser Ophthalmoscopy.

ABSTRACT:

Purpose

To quantify bleaching-induced changes in fundus reflectance in the mouse retina.

Methods

Light reflected from the fundus of albino (Balb/c) and pigmented (C57Bl/6J) mice was measured with a multichannel scanning laser ophthalmoscopy optical coherence tomography (SLO-OCT) optical system. Serial scanning of small retinal regions was used for bleaching rhodopsin and measuring reflectance changes.

Results

Serial scanning generated a saturating reflectance increase centered at 501 nm with a photosensitivity of 1.4 × 10-8 per molecule μm2 in both strains, 2-fold higher than expected were irradiance at the rod outer segment base equal to that at the retinal surface. The action spectrum of the reflectance increase corresponds to the absorption spectrum of mouse rhodop

SUBMITTER: Zhang P 

PROVIDER: S-EPMC4959838 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature