Both BMP4 and serum have significant roles in differentiation of embryonic stem cells to primitive and definitive endoderm.

ABSTRACT: Differentiation of embryonic stem (ES) cells is a heterogeneous process which is influenced by different parameters, including growth and differentiation factors. The aim of the present study was to investigate the effect of bone morphogenetic protein-4 (BMP4) signaling on differentiation of mouse ES cells to endodermal lineages. For this purpose, differentiation of the ES cells was induced by embryoid body (EB) formation through hanging drop method. During the suspension stage, EBs were treated with BMP4 in a medium containing either fetal bovine serum (FBS) or knockout serum replacement (KoSR). After plating, EBs showed differentiation to a heterogeneous population of specialized cell types. Two weeks after plating, all the experimental groups expressed three germ layer markers and some

SUBMITTER: Taha MF 

PROVIDER: S-EPMC4960179 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature