Antibiotic Susceptibility Testing of the Gram-Negative Bacteria Based on Flow Cytometry.

ABSTRACT: Rapidly treating infections with adequate antibiotics is of major importance. This requires a fast and accurate determination of the antibiotic susceptibility of bacterial pathogens. The most frequently used methods are slow because they are based on the measurement of growth inhibition. Faster methods, such as PCR-based detection of determinants of antibiotic resistance, do not always provide relevant information on susceptibility, particularly that which is not genetically based. Consequently, new methods, such as the detection of changes in bacterial physiology caused by antibiotics using flow cytometry and fluorescent viability markers, are being explored. In this study, we assessed whether Alexa Fluor® 633 Hydrazide (AFH), which targets carbonyl groups, can be used for antibiotic susc

SUBMITTER: Saint-Ruf C 

PROVIDER: S-EPMC4960253 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature