A single amino acid substitution alter antigenicity of Glycosylated protein 4 of HP-PRRSV.

ABSTRACT:

Background

Porcine reproductive and respiratory syndrome (PRRS) is an important pig endemic disease in pork-producing countries worldwide. The etiology, porcine reproductive and respiratory syndrome virus (PRRSV), is characterized by fast antigen variability. Glycosylated protein 4 (GP4) is a minor protein in PRRSV virion, but contributes to induce protective immune responses. However, the antigenic characterization of PRRSV GP4 and the role of the mutations in this protein in PRRSV evolution are not clear.

Methods

Peptides chip scanning and peptide based ELISA was used to analyze the antigenic characterization of HP-PRRSV GP4. A total of 142 peptides printed on a chip were used to reveal the antigen reaction characteristics of the HP-PRRSV. The reactions of these peptides w

SUBMITTER: Wang X 

PROVIDER: S-EPMC4960902 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature