Gut Microbial Alterations Associated With Protection From Autoimmune Uveitis.

ABSTRACT:

Purpose

To investigate the contribution of the gut microbiota to the pathogenesis of uveitis.

Methods

Experimental autoimmune uveitis (EAU) in B10.RIII mice was induced using interphotoreceptor binding protein peptide. Mice were treated with oral or intraperitoneal (IP) antibiotics. Effector (Teff) and regulatory (Treg) T lymphocytes were identified using flow cytometry; 16S rRNA gene sequencing and qPCR were performed on gastrointestinal (GI) contents.

Results

Broad-spectrum (four antibiotics given simultaneously) oral, but not IP, antibiotics reduced mean uveitis clinical scores significantly compared with water-treated animals (0.5 vs. 3.0, P < 0.0001 for oral; 3.4 vs. 3.4, P > 0.99 for IP). Both oral metronidazole (P = 0.02) and vancomycin (P < 0.0001) alone decre

SUBMITTER: Nakamura YK 

PROVIDER: S-EPMC4960998 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature