MiRNA Profile in Three Different Normal Human Ocular Tissues by miRNA-Seq.

ABSTRACT:

Purpose

Because microRNAs (miRNAs) have been associated with eye diseases, our study aims to profile ocular miRNA expression in normal human ciliary body (CB), cornea, and trabecular meshwork (TM) using miRNA-Seq to provide a foundation for better understanding of miRNA function and disease involvement in these tissues.

Methods

Total RNAs were extracted from seven normal human CB, seven cornea, and seven TM samples using mirVana total RNA isolation kit. miRNA-Seq was done with Illumina MiSeq. Bowtie software was used to trim and align generated sequence reads, and only exact matches to mature miRNAs from miRBase were included. The miRTarBase database was used to analyze miRNA target interactions, and the expression of five selected miRNAs was validated using droplet digital

SUBMITTER: Drewry M 

PROVIDER: S-EPMC4961003 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature