Validation of Suitable Reference Genes for Assessing Gene Expression of MicroRNAs in Lonicera japonica.

ABSTRACT: MicroRNAs (miRNAs), which play crucial regulatory roles in plant secondary metabolism and responses to the environment, could be developed as promising biomarkers for different varieties and production areas of herbal medicines. However, limited information is available for miRNAs from Lonicera japonica, which is widely used in East Asian countries owing to various pharmaceutically active secondary metabolites. Selection of suitable reference genes for quantification of target miRNA expression through quantitative real-time (qRT)-PCR is important for elucidating the molecular mechanisms of secondary metabolic regulation in different tissues and varieties of L. japonica. For precise normalization of gene expression data in L. japonica, 16 candidate miRNAs were examined in three tissues, as

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4961011 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature