Characterization of Senescence of Culture-expanded Human Adipose-derived Mesenchymal Stem Cells.

ABSTRACT:

Background and objectives

Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates in regenerative medicine. The need for in vitro propagation to obtain therapeutic quantities of the cells imposes a risk of impaired functionality due to cellular senescence. The aim of the study was to analyze in vitro senescence of previously cryopreserved human ADSCs subjected to serial passages in cell culture.

Methods and results

ADSC cultures from 8 donors were cultivated until proliferation arrest was reached. A gradual decline of ADSC fitness was observed by altered cell morphology, loss of proliferative, clonogenic and differentiation abilities and increased β-galactosidase expression all of which occurred in a donor-specific manner. Relative telomere length (RTL) analy

SUBMITTER: Legzdina D 

PROVIDER: S-EPMC4961112 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature