Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation.

ABSTRACT:

Background

SCAR/WAVE is a principal regulator of pseudopod growth in crawling cells. It exists in a stable pentameric complex, which is regulated at multiple levels that are only beginning to be understood. SCAR/WAVE is phosphorylated at multiple sites, but how this affects its biological activity is unclear. Here we show that dephosphorylation of Dictyostelium SCAR controls normal pseudopod dynamics.

Results

We demonstrate that the C-terminal acidic domain of most Dictyostelium SCAR is basally phosphorylated at four serine residues. A small amount of singly phosphorylated SCAR is also found. SCAR phosphorylation site mutants cannot replace SCAR's role in the pseudopod cycle, though they rescue cell size and growth. Unphosphorylatable SCAR is hyperactive-excessive recruitmen

SUBMITTER: Ura S 

PROVIDER: S-EPMC4961229 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature